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KMID : 0191120170320020343
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Journal of Korean Medical Science
2017 Volume.32 No. 2 p.343 ~ p.351
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Nestin Expression in the Adult Mouse Retina with Pharmaceutically Induced Retinal Degeneration
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Moon Chan-Hee
Cho Hee-Yoon
Kim Yoon-Kyung
Park Tae-Kwann
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Abstract
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The present study investigated the temporal pattern and cellular localization of nestin in the adult mouse retina with pharmaceutically induced retinal degeneration using N-methyl-N-nitrosourea (MNU). After a single intraperitoneal injection of MNU in 8-week-old C57BL/6 mice, the animals were sacrificed at 1, 3, 5, 7, and 21 days (n = 6, in each stage). The eyes were examined by means of immunohistochemical tests using nestin, ionized calcium-binding adaptor molecule (Iba-1), CD11b, F4/80, and glial fibrillary acidic protein (GFAP). Western blot analysis and manual cell counting were performed for quantification. Nestin expression was increased after MNU administration. Nestin+/Iba-1+ cells were migrated into outer nuclear layer (ONL) and peaked at day 3 post injection (PI). Nestin+/CD11b+ cells were also mainly identified in ONL at day 3 PI and peaked at day 5. Nestin+/F4/80+ cells were shown in the subretinal space and peaked at day 3 PI. Nestin+/GFAP+ cells were distinctly increased at day 1 PI and peaked at day 5 PI. The up-regulation of nestin expression after MNU administration in adult mouse retinal microglia, and monocyte/macrophage suggests that when retinal degeneration progresses, these cells may revert to a more developmentally immature state. Muller cells also showed reactive gliosis and differentiational changes.
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KEYWORD
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Nestin, N-methyl-N-nitrosourea (MNU), Retinal Degeneration, Mouse
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